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LGALS1/Galectin 1 ELISA Kit

LGALS1 Reaktivität: Maus AA 2-135 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN921097
  • Target Alle LGALS1/Galectin 1 (LGALS1) ELISA Kits anzeigen
    LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
    Bindungsspezifität
    AA 2-135
    Reaktivität
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    156-10000 pg/mL
    Untere Nachweisgrenze
    156 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse GALECTIN-1
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: A2-E135
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <5pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: A2-E135
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Contains 1 galectin domain.

    Plattentyp
    Pre-coated
    Protokoll
    mouse Galectin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Galectin-1 has been precoated onto 96-well plates. Standards(E.coli, A2-E135) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Galectin-1 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Galectin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Galectin-1 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 501, Standard deviation: 22.04, CV(%): 4.4
    • Sample 2: n=16, Mean(pg/ml): 3235, Standard deviation: 148.8, CV(%): 4.6
    • Sample 3: n=16, Mean(pg/ml): 5618, Standard deviation: 309, CV(%): 5.5,
    • Sample 1: n=24, Mean(pg/ml): 521, Standard deviation: 30.22, CV(%): 5.8
    • Sample 2: n=24, Mean(pg/ml): 3547, Standard deviation: 223.5, CV(%): 6.3
    • Sample 3: n=24, Mean(pg/ml): 5296, Standard deviation: 397.2, CV(%): 7.5
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle LGALS1/Galectin 1 (LGALS1) ELISA Kits anzeigen
    LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
    Andere Bezeichnung
    LGALS1 (LGALS1 Produkte)
    Synonyme
    GAL1 ELISA Kit, GBP ELISA Kit, Galectin-1 ELISA Kit, LGAS1 ELISA Kit, LGALS1 ELISA Kit, MGC64502 ELISA Kit, GB10026 ELISA Kit, AA410090 ELISA Kit, Gal-1 ELISA Kit, Galbp ELISA Kit, L-14.5 ELISA Kit, L14 ELISA Kit, Lect14 ELISA Kit, galectin-1 ELISA Kit, Galaptin ELISA Kit, galectin 1 ELISA Kit, galectin-1 ELISA Kit, lectin, galactoside-binding, soluble, 1 L homeolog ELISA Kit, protein enabled ELISA Kit, lectin, galactose binding, soluble 1 ELISA Kit, LGALS1 ELISA Kit, Lgals1 ELISA Kit, CC1G_05003 ELISA Kit, CC1G_05004 ELISA Kit, lgals1.L ELISA Kit, LOC408848 ELISA Kit
    Hintergrund

    Protein Function: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity. Strong inducer of T-cell apoptosis (By similarity). .

    Background: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse. They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.

    Synonyms: Galectin-1,Gal-1,14 kDa lectin,Beta-galactoside-binding lectin L-14-I,Galaptin,Lactose-binding lectin 1,Lectin galactoside-binding soluble 1,S-Lac lectin 1,Lgals1,Gbp,

    Full Gene Name: Galectin-1

    Cellular Localisation: Secreted, extracellular space, extracellular matrix.
    Gen-ID
    16852
    UniProt
    P16045
    Pathways
    Carbohydrate Homeostasis
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