BMP5 ELISA Kit

Produktnummer ABIN921053

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human BMP5.

Kurzübersicht für BMP5 ELISA Kit (ABIN921053)

Target: BMP5 (Bone Morphogenetic Protein 5 (BMP5))

Bindungsspezifität: AA 317-454

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Osseous Tissue

Produktdetails BMP5 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human BMP-5

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: A317-H454

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Homologie: Bovine,Canine,Horse,Monkey,Rabbit

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A317-H454

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the TGF-beta family.

Tissue Specificity: Expressed in the lung and liver.

Plattentyp: Pre-coated

Protokoll: human BMP-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-5 has been precoated onto 96-well plates. Standards(NSO, A317-H454) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-5 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human BMP-5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or bone tissue to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human BMP-5 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 1.23, Standard deviation: 0.059, CV(%): 4.8
• Sample 2: n=16, Mean(ng/ml): 3.46, Standard deviation: 0.180, CV(%): 5.2
• Sample 3: n=16, Mean(ng/ml): 6.92, Standard deviation: 0.388, CV(%): 5.6,
• Sample 1: n=24, Mean(ng/ml): 1.38, Standard deviation: 0.073, CV(%): 5.3
• Sample 2: n=24, Mean(ng/ml): 3.57, Standard deviation: 0.203, CV(%): 5.7
• Sample 3: n=24, Mean(ng/ml): 6.85, Standard deviation: 0.425, CV(%): 6.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu BMP5

Target: BMP5 (Bone Morphogenetic Protein 5 (BMP5))

Andere Bezeichnung: BMP5

Hintergrund:

Protein Function: Induces cartilage and bone formation.

Background: Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on human chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.

Synonyms: Bone morphogenetic protein 5,BMP-5,BMP5,

Full Gene Name: Bone morphogenetic protein 5

Cellular Localisation: Secreted.

Gen-ID: 653

UniProt: P22003

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process