Angiogenin ELISA Kit (Angiogenin, Ribonuclease, RNase A Family, 5)

Details for Product ANG ELISA Kit No. ABIN921052, Anbieter: Anmelden zum Anzeigen
Antigen
  • ALS9
  • HEL168
  • RNASE4
  • RNASE5
  • ANG
  • AI385586
  • Ang1
  • Rnase5
  • Rnase5a
  • ANG1
  • angiogenin
  • angiogenin, ribonuclease, RNase A family, 5
  • ANG
  • Ang
Epitop
AA 25-147
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
31
16
13
9
6
5
4
3
3
3
2
2
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
78-5000 pg/mL
Untere Nachweisgrenze
78 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ANG
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Analytische Methode Quantitative
Nachweismethode Colorimetric
Immunogen Expression system for standard: E.coli
Immunogen sequence: Q25-P147
Spezifität Expression system for standard: E.coli
Immunogen sequence: Q25-P147
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <12pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Plasmids, Primers & others Plasmide, Primers & weitere Angiogenin products on genomics-online (e.g. as negative or positive controls)
Andere Bezeichnung ANG (ANG ELISA Kit Abstract)
Hintergrund

Protein Function: Binds to actin on the surface of endothelial cells, once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. .

Background: Angiogenin(Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al.(2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al.(1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

Synonyms: Angiogenin,3.1.27.-,Ribonuclease 5,RNase 5,ANG,RNASE5,

Full Gene Name: Angiogenin

Cellular Localisation: Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.
Gen-ID 283
UniProt P03950
Applikations-hinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Sequence similarities: Belongs to the pancreatic ribonuclease family.

Tissue Specificity: Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. .

Plattentyp Pre-coated
Protokoll human ANG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANG has been precoated onto 96-well plates. Standards(E.coli, Q25-P147) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ANG amount of sample captured in plate.
Testdurchführung

Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human ANG standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ANG standard solution and each sample be measured in duplicate.

Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 312, Standard deviation: 9.4, CV(%): 3
  • Sample 2: n=16, Mean(pg/ml): 672, Standard deviation: 23.52, CV(%): 3.5
  • Sample 3: n=16, Mean(pg/ml): 1663, Standard deviation: 54.9, CV(%): 3.3,
  • Sample 1: n=24, Mean(pg/ml): 635, Standard deviation: 45.72, CV(%): 7.2
  • Sample 2: n=24, Mean(pg/ml): 1856, Standard deviation: 165.2, CV(%): 8.9
  • Sample 3: n=24, Mean(pg/ml): 3489, Standard deviation: 300, CV(%): 8.6
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months
Bilder des Herstellers
ELISA image for Angiogenin, Ribonuclease, RNase A Family, 5 (ANG) ELISA Kit (ABIN921052) Human ANG PicoKine ELISA Kit standard curve
Produkt verwendet in: Shi, Zhang, Fu, Lu, Ye, Xiao: "Angiotensin II as a morphogenic cytokine stimulating fibrogenesis of human tenon's capsule fibroblasts." in: Investigative ophthalmology & visual science, Vol. 56, Issue 2, pp. 855-64, 2015 (PubMed).

Bai, Li, Tian, Zhou: "Antiangiogenic treatment diminishes renal injury and dysfunction via regulation of local AKT in early experimental diabetes." in: PLoS ONE, Vol. 9, Issue 4, pp. e96117, 2014 (PubMed).

Li, Fan, Song, Zhang, Chen, Li, Mi, Ma, Song, Tao, Li: "Expression of angiopoietin-2 and vascular endothelial growth factor receptor-3 correlates with lymphangiogenesis and angiogenesis and affects survival of oral squamous cell carcinoma." in: PLoS ONE, Vol. 8, Issue 9, pp. e75388, 2013 (PubMed).

Allgemeine Veröffentlichungen Liu, Miao, Sheng, Xie, Huang, Shan, Yang: "Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing." in: Journal of Zhejiang University. Science. B, Vol. 11, Issue 1, pp. 10-6, 2009 (PubMed).

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