Osteopontin ELISA Kit

Produktnummer ABIN8126238

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Osteopontin.

Kurzübersicht für Osteopontin ELISA Kit (ABIN8126238)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Osteopontin ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: This assay employs an antibody specific for Anti-human OPN coated on a 96-well plate.

Analytische Methode: Quantitative

Sensitivität: 93.75 pg/mL

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Human OPN concentrations in serum, plasma.  Detection Range: 0.156 - 10 ng/mL Sensitivity: < 93.75 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-human OPN coated on a 96-well plate. Standards and samples are pipetted into the wells and OPN present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human OPN antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of OPN bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: Please refer to the Manual

Details zu Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Andere Bezeichnung: OPN (Osteopontin)

Pathways: Regulation of Cell Size