CXCL9 ELISA Kit

Produktnummer ABIN8126235

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human CXCL9.

Kurzübersicht für CXCL9 ELISA Kit (ABIN8126235)

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails CXCL9 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytische Methode: Quantitative

Sensitivität: 94 pg/mL

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Human CXCL9 concentrations in serum, plasma. Detection Range: 156 - 10,000 pg/mL Sensitivity: <94 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human CXCL9 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human CXCL9 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CXCL9 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human CXCL9 can be calculated using the standard curve.  

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: Please refer to the Manual

Details zu CXCL9

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Andere Bezeichnung: CXCL9/MIG (C-X-C motif chemokine 9)