NPY ELISA Kit

Produktnummer ABIN8091125

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Huhn NPY.

Kurzübersicht für NPY ELISA Kit (ABIN8091125)

Target: NPY (Neuropeptide Y (NPY))

Reaktivität: Huhn

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Produktdetails NPY ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Verwendungszweck: Chicken NPY (Neuropeptide Y) ELISA Kit

Analytische Methode: Quantitative

Spezifität: Specifically binds with NPY , no obvious cross reaction with other analogues.

Sensitivität: 18.75 pg/mL

Bestandteile:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Benötigtes Material:

• Microplate reader (wavelength: 450nm)
• Microorifice oscillator
• or shake bed 37 °C incubator (When using a water bath incubator
• ensure the internal air temperature is 35-38 °C, When using the cell CO2 incubator
• use a sealed bag to isolate the microplate)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Sandwich ELISA, Double Antibody

Probenmenge: 100 μL

Testdauer: 4 h

Plattentyp: Pre-coated

Protokoll: Step 1: Add 100 µL standard or sample into each well, Seal the plate and incubate on a microorifice oscillator or shake bed at 200 rpm and 2-8 °C for 2 hours with shaking, ensuring no spillage from any well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Washing: Wash the plate twice without immersion. Step 2: Add 100 µL biotin-labeled antibody working solution into each well, Seal the plate and incubate on a microorifice oscillator or shake bed at 200 rpm and room temperature (25 °C ± 3 °C) for 1 hours with shaking, ensuring no spillage from any well. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL SABC working solution into each well, Seal the plate and incubate on a microorifice oscillator or shake bed at 200 rpm and room temperature (25 °C ± 3 °C) for 0.5 hours with shaking, ensuring no spillage from any well. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and static incubate for 30 minutes at room temperature (25 °C ± 3 °C). (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid direct light exposure to antibody solutions

Lagerung: 4 °C

Informationen zur Lagerung: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Haltbarkeit: 12 months

Details zu NPY

Target: NPY (Neuropeptide Y (NPY))

Andere Bezeichnung: NPY

Hintergrund: Neuropeptide Y, Neuropeptide tyrosine, NPY, NPY

UniProt: P28673

Pathways: Feeding Behaviour