Lipopolysaccharides (LPS) ELISA Kit
Kurzübersicht für Lipopolysaccharides (LPS) ELISA Kit (ABIN8090869)
Target
Alle Lipopolysaccharides (LPS) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 0.313 μg/mL
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Verwendungszweck
- Mouse LPS (Lipopolysaccharides) ELISA Kit
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Analytische Methode
- Quantitative
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Spezifität
- Specifically binds with LPS , no obvious cross reaction with other analogues.
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Sensitivität
- 0.188 μg/mL
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Benötigtes Material
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- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
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Lipopolysaccharides (LPS) ELISA Kit
Reaktivität: Diverse Spezies Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitReaktivität: Diverse Spezies Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitReaktivität: Diverse Spezies Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitReaktivität: Human Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitReaktivität: Ratte Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitReaktivität: Rind (Kuh) Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitReaktivität: Maus Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitSmall Sample Reaktivität: Diverse Spezies Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitQuick Reaktivität: Diverse Spezies Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitHigh Sensitivity Reaktivität: Diverse Spezies Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Kommentare
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Competitive ELISA, Coated with Antibody
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Testdauer
- 2 h
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Plattentyp
- Pre-coated
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Protokoll
- Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 100 µL standard/sample/biotin-labeled antigen working solution into each well. Seal the plate and statically incubate for 45 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution into each well, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- Avoid direct light exposure to antibody solutions
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
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Haltbarkeit
- 12 months
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- Lipopolysaccharides (LPS)
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Andere Bezeichnung
- LPS
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Substanzklasse
- Chemical
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Hintergrund
- LPS, Lipopolysaccharides
Target Alle Lipopolysaccharides (LPS) ELISA Kits anzeigen
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