P4HA3 ELISA Kit

Produktnummer ABIN8085713

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human P4HA3.

Kurzübersicht für P4HA3 ELISA Kit (ABIN8085713)

Target: P4HA3 (Prolyl 4-Hydroxylase, alpha Polypeptide III (P4HA3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Produktdetails P4HA3 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: Human Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) ELISA Kit for detecting P4Ha3 in tissue homogenates

Analytische Methode: Quantitative

Sensitivität: 0.066 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu P4HA3

Target: P4HA3 (Prolyl 4-Hydroxylase, alpha Polypeptide III (P4HA3))

Andere Bezeichnung: Prolyl-4-Hydroxylase Alpha Polypeptide III

Hintergrund: Gene Aliases: Procollagen-Proline,2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase) ,Alpha Polypeptide III

Gen-ID: 283208

UniProt: Q7Z4N8