Pituitary Adenylate Cyclase Activating Peptide ELISA Kit

Produktnummer ABIN8085229

Human Pituitary Adenylate Cyclase Activating Peptide ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Pituitary Adenylate Cyclase Activating Peptide.

Kurzübersicht für Pituitary Adenylate Cyclase Activating Peptide ELISA Kit (ABIN8085229)

Target: Pituitary Adenylate Cyclase Activating Peptide

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 6.17 pg/mL - 500 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails

Untere Nachweisgrenze: 6.17 pg/mL

Verwendungszweck: Human Pituitary Adenylate Cyclase Activating Peptide (PACAP) ELISA Kit for detecting PACAP in serum, plasma, tissue homogenates

Analytische Methode: Quantitative

Sensitivität: 2.92 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Antigendetails

Target: Pituitary Adenylate Cyclase Activating Peptide

Hintergrund: Gene Aliases: ADCYAP1, PRP-48, Pituitary Adenylate Cyclase Activating Peptide

Gen-ID: 116

UniProt: P18509