PIK3C2A ELISA Kit

Produktnummer ABIN8085154

Maus PIK3C2A ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus PIK3C2A.

Kurzübersicht für PIK3C2A ELISA Kit (ABIN8085154)

Target: PIK3C2A (Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Produktdetails PIK3C2A ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: Mouse Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) ELISA Kit for detecting PIK3C2a in tissue homogenates

Analytische Methode: Quantitative

Sensitivität: 0.057 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu PIK3C2A

Target: PIK3C2A (Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A))

Andere Bezeichnung: Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide

Hintergrund: Gene Aliases: PI3K2a, CPK, PI3-K-C2 (Alpha) , PI3-K-C2A, PI3K2-a, Phosphoinositide 3-Kinase 2a, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

Gen-ID: 18704

UniProt: Q61194

Pathways: EGFR Signaling Pathway, Inositol Metabolic Process, Platelet-derived growth Factor Receptor Signaling