PDE1A ELISA Kit

Produktnummer ABIN8085114

Das Maus PDE1A ELISA Kit (ABIN8085114) ist ein Colorimetric ELISA Kit zur Detektion von Maus PDE1A.

Kurzübersicht für PDE1A ELISA Kit (ABIN8085114)

Target: PDE1A (Phosphodiesterase 1A (PDE1A))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Produktdetails PDE1A ELISA Kit

Untere Nachweisgrenze: 0.312 ng/mL

Verwendungszweck: Mouse Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) ELISA Kit for detecting PDE1A in tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Sensitivität: 0.124 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu PDE1A

Target: PDE1A (Phosphodiesterase 1A (PDE1A))

Andere Bezeichnung: Phosphodiesterase 1A, Calmodulin Dependent

Hintergrund: Gene Aliases: hCam-1, 61 kDa Cam-PDE, Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A

Gen-ID: 18573

UniProt: Q61481

Pathways: Neurotrophin Signalübertragung, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma