Intestinal Alkaline Phosphatase ELISA Kit

Produktnummer ABIN8082337

Human Intestinal Alkaline Phosphatase ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Intestinal Alkaline Phosphatase.

Kurzübersicht für Intestinal Alkaline Phosphatase ELISA Kit (ABIN8082337)

Target: Intestinal Alkaline Phosphatase (ALPI)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails Intestinal Alkaline Phosphatase ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Verwendungszweck: Human Alkaline Phosphatase, Intestinal (ALPI) ELISA Kit for detecting ALPI in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Sensitivität: 0.63 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Andere Bezeichnung: Alkaline Phosphatase, Intestinal

Hintergrund: Gene Aliases: CIP, IAP, Calf Intestinal Alkaline Phosphatase

Gen-ID: 248

UniProt: P09923