Inhibitory Subunit Of NF kappa B alpha ELISA Kit

Produktnummer ABIN8081100

Das Human Inhibitory Subunit Of NF kappa B alpha ELISA Kit (ABIN8081100) ist ein Colorimetric ELISA Kit zur Detektion von Human Inhibitory Subunit Of NF kappa B alpha.

Kurzübersicht für Inhibitory Subunit Of NF kappa B alpha ELISA Kit (ABIN8081100)

Target: Inhibitory Subunit Of NF kappa B alpha

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) High-sensitive ELISA Kit for detecting IkBa in tissue homogenates, cell lysates

Analytische Methode: Quantitative

Sensitivität: 11.4 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Antigendetails

Target: Inhibitory Subunit Of NF kappa B alpha

Hintergrund: Gene Aliases: NFKBIA, IKB-A, MAD-3, NFKBI, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor,Alpha, Major histocompatibility complex enhancer-binding protein MAD3

Gen-ID: 4792

UniProt: P25963