HSD17B1 ELISA Kit

Produktnummer ABIN8080827

Das Human HSD17B1 ELISA Kit (ABIN8080827) ist ein Colorimetric ELISA Kit zur Detektion von Human HSD17B1.

Kurzübersicht für HSD17B1 ELISA Kit (ABIN8080827)

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails HSD17B1 ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Verwendungszweck: Human 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) ELISA Kit for detecting HSD17b1 in tissue homogenates, cell lysates

Analytische Methode: Quantitative

Sensitivität: 0.55 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu HSD17B1

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Andere Bezeichnung: 17-Beta-Hydroxysteroid Dehydrogenase Type 1

Hintergrund: Gene Aliases: EDH17B2, EDHB17, HSD17, SDR28C1, Estradiol 17-Beta-Dehydrogenase 1, Short Chain Dehydrogenase/Reductase Family 28CE,1, 20 alpha-hydroxysteroid dehydrogenase

Gen-ID: 3292

UniProt: P14061

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis