HLA-E ELISA Kit

Produktnummer ABIN8080727

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human HLA-E.

Kurzübersicht für HLA-E ELISA Kit (ABIN8080727)

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6.25 pg/mL - 400 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails HLA-E ELISA Kit

Untere Nachweisgrenze: 6.25 pg/mL

Verwendungszweck: Human Major Histocompatibility Complex Class I E (MHCE) ELISA Kit for detecting MHCE in serum, plasma, tissue homogenates

Analytische Methode: Quantitative

Sensitivität: 2.27 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu HLA-E

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Andere Bezeichnung: Major Histocompatibility Complex Class I E

Hintergrund: Gene Aliases: HLAE, EA1.2, EA2.1, HLA-6.2, MHC-E, HLA-E, Leukocyte Antigen E, MHC class I antigen E, HLA class I histocompatibility antigen, alpha chain E

Gen-ID: 3133

UniProt: P13747

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response