Estrogen Receptor alpha ELISA Kit

Produktnummer ABIN8078712

Das Ratte Estrogen Receptor alpha ELISA Kit (ABIN8078712) ist ein Colorimetric ELISA Kit zur Detektion von Ratte Estrogen Receptor alpha.

Kurzübersicht für Estrogen Receptor alpha ELISA Kit (ABIN8078712)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails Estrogen Receptor alpha ELISA Kit

Untere Nachweisgrenze: 0.312 ng/mL

Verwendungszweck: Rat Estrogen Receptor Alpha (ERa) ELISA Kit for detecting ERa in serum, plasma, tissue homogenates

Analytische Methode: Quantitative

Sensitivität: 0.138 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Andere Bezeichnung: Estrogen Receptor Alpha

Hintergrund: Gene Aliases: ER-A1, ER, ESR, ESR1, ESRA, NR3A1, NR3-A1, Estrogen Receptor 1, Nuclear Receptor Subfamily 3,Group A,Member 1

Gen-ID: 24890

UniProt: P06211

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly