Cofilin CLIA Kit

Produktnummer ABIN8077055

Dieses Chemiluminescent ELISA kit dient der quantitativen Messung von Human Cofilin.

Kurzübersicht für Cofilin CLIA Kit (ABIN8077055)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 13.7 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Produktdetails Cofilin CLIA Kit

Untere Nachweisgrenze: 13.7 pg/mL

Verwendungszweck: Human Cofilin 1 (CFL1) CLIA Kit for detecting CFL1 in tissue homogenates

Analytische Methode: Quantitative

Sensitivität: 6.3 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Antigendetails

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Andere Bezeichnung: Cofilin 1

Hintergrund: Gene Aliases: CFL, p18, N-Cofilin, 18 kDa phosphoprotein, Cofilin 1, Non Muscle

Gen-ID: 1072

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events