iPLA2 ELISA Kit

Produktnummer ABIN8075633

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Ratte iPLA2.

Kurzübersicht für iPLA2 ELISA Kit (ABIN8075633)

Target: iPLA2 (Calcium Independent Phospholipase A2 (iPLA2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails iPLA2 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: Rat Phospholipase A2, Calcium Independent (iPLA2) ELISA Kit for detecting iPLA2 in serum, plasma

Analytische Methode: Quantitative

Sensitivität: 6.6 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu iPLA2

Target: iPLA2 (Calcium Independent Phospholipase A2 (iPLA2))

Andere Bezeichnung: Phospholipase A2, Calcium Independent

Hintergrund: Gene Aliases: PLA2G6, CaI-PLA2, GVI, INAD1, PNPLA9, Ca2+ Independent PLA2, 85 kDa Calcium-Independent Phospholipase A2, Phospholipase A2,group VI (Cytosolic,Calcium-Independent)

Gen-ID: 360426

UniProt: P97570