ALPL CLIA Kit

Produktnummer ABIN8074325

Dieses Chemiluminescent ELISA kit dient der quantitativen Messung von Ratte ALPL.

Kurzübersicht für ALPL CLIA Kit (ABIN8074325)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reaktivität: Ratte

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.27 ng/mL - 200 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum

Produktdetails ALPL CLIA Kit

Untere Nachweisgrenze: 0.27 ng/mL

Verwendungszweck: Rat ALPL CLIA kit for detecting Alkaline Phosphatase, Tissue-nonspecific in Serum, Plasma, Cell Culture Supernates, Cell Lysates

Analytische Methode: Quantitative

Sensitivität: 0.12 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Antigendetails

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Andere Bezeichnung: Alkaline Phosphatase, Tissue-nonspecific

Hintergrund: Gene Aliases: BALP, BSAP, HOPS, AP-TNAP, TNSALP, Bone Alkaline Phosphatase, Alkaline Phosphatase,Tissue-Nonspecific Isozyme, Alkaline Phosphatase, Liver/Bone/Kidney

Gen-ID: 25586

UniProt: P08289