beta Actin ELISA Kit

Produktnummer ABIN8073938

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human, Ratte und Maus beta Actin.

Kurzübersicht für beta Actin ELISA Kit (ABIN8073938)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reaktivität: Human, Ratte, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Produktdetails beta Actin ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Verwendungszweck: Human/Mouse/Rat Beta Actin (ACTB) ELISA Kit for detecting ACTB in tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Sensitivität: 0.55 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: Beta Actin

Hintergrund: Gene Aliases: Actin Beta, ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed

Gen-ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction