Thyroxine T4 ELISA Kit

Produktnummer ABIN8071049

Human Thyroxine T4 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Thyroxine T4.

Kurzübersicht für Thyroxine T4 ELISA Kit (ABIN8071049)

Target: Thyroxine T4 (T4)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.494 ng/mL - 10000 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum, Urine

Produktdetails Thyroxine T4 ELISA Kit

Untere Nachweisgrenze: 0.494 ng/mL

Verwendungszweck: T4 Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting T4 based on the competitive enzyme immunoassay principle

Analytische Methode: Quantitative

Spezifität: This EIA kit detects human Thyroxine / T4

Bestandteile:

Size / Description
• T4 Microplate
• Assay Diluent
• Standard T4
• Positive Control
• Anti-T4 Antibody
• 10 μL
• HRP-conjugated secondary antibody
• 5 μL
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• GraphPad Prism or SigmaPlot software (or other software which can perform four-parameter logistic regression models)
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• Aluminum foil
• Plastic wrap

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2-fold

Probenmenge: 50 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
3. Add 50 μL anti-T4 to appropriate well. Incubate 1 hour at room temperature. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 2-10 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Details zu Thyroxine T4

Target: Thyroxine T4 (T4)

Andere Bezeichnung: Thyroxine

Substanzklasse: Amino Acid

Hintergrund: Optional[Gene Symbols]: Thyroxine (T4), the primary hormone produced by the thyroid gland, plays a vital role in regulating metabolism, growth, and development. This hormone is synthesized from tyrosine residues in thyroglobulin and contains four iodine atoms, making it a key component of iodine metabolism in the body. T4 exerts its effects by being converted into the more active triiodothyronine (T3), which influences numerous cellular processes by binding to thyroid hormone receptors.

Research has highlighted the critical involvement of thyroxine in the pathogenesis of various diseases, including hypothyroidism, hyperthyroidism, cardiovascular disorders, and metabolic syndromes. Dysregulation of T4 levels affects energy homeostasis, thermogenesis, and tissue differentiation, with far-reaching impacts on nearly every organ system. Moreover, thyroid hormone imbalances have been linked to alterations in other endocrine pathways, emphasizing the interconnected nature of T4 in maintaining physiological and metabolic equilibrium.

The T4 ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of T4 in serum, plasma, cell supernatant or urine samples collected from humans. The quantity of T4 is determined by a known T4 standard curve. This kit has a high sensitivity of approximately 0.494 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.