tau ELISA Kit

Produktnummer ABIN8070982

Das Human, Maus und Ratte tau ELISA Kit (ABIN8070982) ist ein Colorimetric ELISA Kit zur Detektion von Human, Maus und Ratte tau.

Kurzübersicht für tau ELISA Kit (ABIN8070982)

Target: tau (tau Protein)

Bindungsspezifität: pThr217

Reaktivität: Human, Maus, Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.37 ng/mL - 90 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Lysate

Produktdetails tau ELISA Kit

Untere Nachweisgrenze: 0.37 ng/mL

Verwendungszweck: Phospho-TAU (Thr217) Quantitative ELISA Kit is a convenient and sensitive assay to detect phosphorylated Tau at Threonine-217 (p-Tau217) in human cell lysate samples.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human, mouse and rat Phospho TAU (Thr217).

Sensitivität: 0.09 ng/mL

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Details zu tau

Target: tau (tau Protein)

Andere Bezeichnung: Tau

Hintergrund:

Gene Symbols: MAPT MAPTL MTBT1 TAU

Protein Name (Synonyms): Microtubule-associated protein tau (Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)

Gen-ID: 4137

UniProt: P10636