REG3A ELISA Kit

Produktnummer ABIN8070631

Das Maus und Ratte REG3A ELISA Kit (ABIN8070631) ist ein Colorimetric ELISA Kit zur Detektion von Maus und Ratte REG3A.

Kurzübersicht für REG3A ELISA Kit (ABIN8070631)

Target: REG3A (Regenerating Islet-Derived 3 alpha (REG3A))

Reaktivität: Maus, Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Produktdetails REG3A ELISA Kit

Verwendungszweck: Rat Reg3A ELISA Kit.

Analytische Methode: Quantitative

Spezifität: The antibody pair provided in this kit recognizes Bovine ASAHL.

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Details zu REG3A

Target: REG3A (Regenerating Islet-Derived 3 alpha (REG3A))

Andere Bezeichnung: Reg3

Hintergrund:

Gene Symbols: Reg3a

Protein Name (Synonyms): Regenerating islet-derived protein 3-alpha, REG-3-alpha, Islet of Langerhans regenerating protein 3, REG 3, Lithostathine 3, Pancreatitis-associated protein 2, RegIII, Regenerating islet-derived protein III-alpha, Reg III-alpha

UniProt: P35231