Nucleoprotein ELISA Kit

Produktnummer ABIN8070296

Human Nucleoprotein ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Nucleoprotein.

Kurzübersicht für Nucleoprotein ELISA Kit (ABIN8070296)

Target: Nucleoprotein

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails

Verwendungszweck: SFTS Virus Nucleoprotein (NP) Human IgA ELISA kit is an in-vitro indirect ELISA for the quantitative measurement of Human IgA autoantibody against NP in serum or plasma samples. This product is for research use only. Not for therapeutic or diagnostic applications.

Analytische Methode: Quantitative

Bestandteile:

• Pre-coated 96-well Microplate
• Wash Buffer
• Positive Control
• Biotinylated Secondary Antibody
• HRP-Streptavidin Concentrate
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent
• Sample Diluent

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL Positive Control or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Secondary Antibody into respective wells. Incubate 1 hour at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigendetails

Target: Nucleoprotein

Substanzklasse: Influenza Protein

Hintergrund:

Background: This < span class="italic-txt">in-vitro ELISA kit employs an indirect ELISA method. In this kit, standard 96-well plates are coated with the pathogen antigen, which combines with the corresponding antibody present in a sample and positive control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgA/G/M antibody is added, a complex forms between the biotinylated secondary antibody and human antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-pathogen antigen antibody present. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The intensity of the resulting color (measured at 450 nm) is used as a calibration curve for interpretation purposes in the different assays.

Protein Name (Synonyms): Nucleoprotein, Nucleocapsid protein

Gen-ID: 13231122

UniProt: F1BA48