Nuclease ELISA Kit

Produktnummer ABIN8070291

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Serratia marcescens Nuclease.

Kurzübersicht für Nuclease ELISA Kit (ABIN8070291)

Target: Nuclease

Reaktivität: Serratia marcescens

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 19.92 pg/mL - 9600 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant

Produktdetails Nuclease ELISA Kit

Untere Nachweisgrenze: 19.92 pg/mL

Verwendungszweck: Nuclease ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of nucleases, including SuperNuclease® and Benzonase®, in cell culture supernatants and purified biological products while nuclease is supplied to remove host cell DNA during manufacturing.

Analytische Methode: Quantitative

Spezifität: This ELISA kit has been validated with SuperNuclease®, Benzonase® and RayBio® Universal Nuclease, and should react with other Serratia marcescens nuclease products but not tested. Both non-denatured and denatured nuclease can be detected by this kit.

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard and sample dilutions

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Details zu Nuclease

Target: Nuclease

Hintergrund:

Gene Symbols: nucA nuc

Protein Name (Synonyms): Nuclease (EC 3.1.30.2) (Endonuclease) [Cleaved into: Nuclease isoform Sm2, Nuclease isoform Sm3, Nuclease isoform Sm1

UniProt: P13717