Notch1 ELISA Kit

Produktnummer ABIN8070276

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Notch1.

Kurzübersicht für Notch1 ELISA Kit (ABIN8070276)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Bindungsspezifität: acetylated, total

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Lysate, Tissue Lysate

Produktdetails Notch1 ELISA Kit

Verwendungszweck: Measures acetylated NOTCH-1 (NOTCH1) and total NOTCH-1 in human cell and tissue lysates, enabling precise assessment of NOTCH-1 acetylation by ELISA. This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.

Analytische Methode: Quantitative

Bestandteile:

Size / Qty
• Microplate Coated With Anti-Pan-NOTCH-1 Antibody
• Anti-Acetylation Detection Antibody
• Anti-NOTCH-1 Detection Antibody
• HRP-Conjugated Streptavidin
• 200 μL
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Log-log graph paper or computer and software for ELISA data analysis
• Absorbent paper
• Tubes to prepare sample dilutions
• Distilled or deionized water
• Shaker

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed.
2. Add 100 μL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Details zu Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Andere Bezeichnung: Notch-1

Hintergrund:

Gene Symbols: NOTCH1, TAN1

Background: The Acetylated-NOTCH-1 and Total-NOTCH-1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human acetylated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan NOTCH-1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and NOTCH-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-acetylation antibody is used to detect acetylated NOTCH-1 or biotinylated anti-NOTCH-1 antibody is used to detect total NOTCH-1 . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of acetylated NOTCH-1 or total NOTCH-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): Neurogenic locus notch homolog protein 1 (Notch 1, hN1), Translocation-associated notch protein TAN-1

Gen-ID: 4851

UniProt: P46531

Pathways: Notch Signalweg, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development