NEFH ELISA Kit

Produktnummer ABIN8070229

Das Human NEFH ELISA Kit (ABIN8070229) ist ein Colorimetric ELISA Kit zur Detektion von Human NEFH.

Kurzübersicht für NEFH ELISA Kit (ABIN8070229)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Bindungsspezifität: phosphorylated

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.2 pg/mL - 40 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Lysate

Produktdetails NEFH ELISA Kit

Untere Nachweisgrenze: 0.2 pg/mL

Verwendungszweck: Phospho-NFH Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human phosphorylated neurofilament heavy polypeptide (NFH) in lysate samples.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human Phospho NFH.

Bestandteile:

• Anti-Pan-NFH Microplate
• Phospho-NFH Standard
• Anti-Phospho NFH Detection Antibody
• HRP-Streptavidin
• Wash Buffer
• Assay Diluent B
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

,

• Anti-Pan-NFH Microplate
• Phospho-NFH Standard
• Anti-Phospho NFH Detection Antibody
• HRP-Streptavidin
• Wash Buffer
• Assay Diluent B
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Log-log graph paper or computer and software for ELISA data analysis
• Absorbent paper
• Tubes to prepare the positive control or sample dilutions
• Distilled or deionized water
• Shaker

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and positive control as instructed.
2. Add 100 μL of each standard and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: NFH

Hintergrund:

Gene Symbols: NEFH, KIAA0845, NFH

Background: Phospho-NFH Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human phosphorylated neurofilament heavy polypeptide (NFH). An anti-pan NFH antibody has been coated onto a 96-well plate. Standard and Samples are pipetted into the wells and NFH present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phospho-NFH (KSP site) antibody is used to detect phosphorylated NFH. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NFH bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): Neurofilament heavy polypeptide (NF-H) (200 kDa neurofilament protein) (Neurofilament triplet H protein)

Gen-ID: 4744

UniProt: P12036