MBP ELISA Kit

Produktnummer ABIN8070008

Human MBP ELISA Kit, Colorimetric Assay für die Quantifizierung von Human MBP.

Kurzübersicht für MBP ELISA Kit (ABIN8070008)

Target: MBP

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails MBP ELISA Kit

Verwendungszweck: Mylein Basic Protein (MBP) Human IgM ELISA Kit is an in-vitro indirect ELISA for the quantitative measurement of Human IgM autoantibody against MBP in serum or plasma samples. This product is for research use only. Not for therapeutic or diagnostic applications.

Analytische Methode: Quantitative

Bestandteile:

• Pre-coated 96-well microplate
• Wash Buffer
• Positive Control
• Biotinylated Detection Antibody
• Biotinylated Anti-Positive Control
• HRP-Streptavidin concentrate
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent
• Sample Diluent

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Details zu MBP

Target: MBP

Hintergrund:

Gene Symbols: MBP

Protein Name (Synonyms): Myelin basic protein (MBP) (Myelin A1 protein) (Myelin membrane encephalitogenic protein)

Gen-ID: 4155

UniProt: P02686