Lipopolysaccharides (LPS) ELISA Kit

Produktnummer ABIN8069969

E. coli Lipopolysaccharides (LPS) ELISA Kit, Colorimetric Assay für die Quantifizierung von E. coli Lipopolysaccharides (LPS).

Kurzübersicht für Lipopolysaccharides (LPS) ELISA Kit (ABIN8069969)

Target: Lipopolysaccharides (LPS)

Reaktivität: E. coli

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.01 ng/mL - 12 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate

Produktdetails Lipopolysaccharides (LPS) ELISA Kit

Untere Nachweisgrenze: 0.01 ng/mL

Verwendungszweck: Escherichia coli Lipopolysaccharide ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of process-related LPS, the common endotoxin impurities, derived from production processes when E. coli strains are utilized.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects E. coli LPS. The possibility of this antibody pair to detect LPS of other gram-negative bacteria has not been tested.

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Details zu Lipopolysaccharides (LPS)

Target: Lipopolysaccharides (LPS)

Andere Bezeichnung: LPS

Substanzklasse: Chemical