SPA ELISA Kit

Produktnummer ABIN8069705

Staphylococcus SPA ELISA Kit, Colorimetric Assay für die Quantifizierung von Staphylococcus SPA.

Kurzübersicht für SPA ELISA Kit (ABIN8069705)

Target: SPA (Immunoglobulin G Binding Protein A (SPA))

Reaktivität: Staphylococcus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 8.92 pg/mL - 7500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate

Produktdetails SPA ELISA Kit

Untere Nachweisgrenze: 8.92 pg/mL

Verwendungszweck: Protein A ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Staphylococcus aureus Protein A and recombinant Protein A contamination in biologics.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects Protein A. Other targets not determined.

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard and sample dilutions

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Details zu SPA

Target: SPA (Immunoglobulin G Binding Protein A (SPA))

Andere Bezeichnung: Staphylococcal protein A

Hintergrund:

Gene Symbols: spa SAOUHSC_00069

Protein Name (Synonyms): Immunoglobulin G-binding protein A (IgG-binding protein A) (Staphylococcal protein A) (SpA)

Gen-ID: 3919448

UniProt: P02976