IgM ELISA Kit

Produktnummer ABIN8069632

Das Human IgM ELISA Kit (ABIN8069632) ist ein Colorimetric ELISA Kit zur Detektion von Human IgM.

Kurzübersicht für IgM ELISA Kit (ABIN8069632)

Target: IgM

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Serum

Produktdetails IgM ELISA Kit

Verwendungszweck: COVID-19 N and S1 RBD protein Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgM antibody against SARS-CoV-2 in serum. This Product is for Research Use Only. Not for use in in vitro diagnostics.

Analytische Methode: Semi-Quantitative

Bestandteile:

• SARS-CoV-2 N-protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Negative Control
• HRP-anti positive control IgM Antibody
• HRP-anti Human IgM Antibody
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent

,

• SARS-CoV-2 N-protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Negative Control
• HRP-anti positive control IgM Antibody
• HRP-anti Human IgM Antibody
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control, negative control, or sample to each well. Incubate 1.5 hours at room temperature.
3. Add 100 μL prepared HRP-anti positive control IgM Antibody and/or 100 μL of prepared HRP-anti Human IgM Antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
5. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -80 °C

Informationen zur Lagerung: The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80^°C.

Details zu IgM

Target: IgM

Substanzklasse: Antibody

Hintergrund: Gene Symbols: IGM