IgG ELISA Kit

Produktnummer ABIN8069260

Das Human IgG ELISA Kit (ABIN8069260) ist ein Colorimetric ELISA Kit zur Detektion von Human IgG.

Kurzübersicht für IgG ELISA Kit (ABIN8069260)

Target: IgG

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Serum

Produktdetails IgG ELISA Kit

Verwendungszweck: COVID-19 Gamma Spike Variant Human IgG ELISA Kit is an antibody detection kit (indirect ELISA method) for the quantitative measurement of human IgG against the spike protein mutations (K417T, E484K, N501Y) associated with the Gamma variant of SARS-CoV-2. This Product is for Research Use Only. Not for use in in vitro diagnostics.

Analytische Methode: Semi-Quantitative

Bestandteile:

• SARS-CoV-2 Protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgG
• HRP-Streptavidin concentrate
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent
• 5X Sample Diluent

,

• SARS-CoV-2 Protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgG
• HRP-Streptavidin concentrate
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent
• 5X Sample Diluent

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Distilled or deionized water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -80 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

Details zu IgG

Target: IgG

Substanzklasse: Antibody

Hintergrund: Gene Symbols: IGG