IgG ELISA Kit

Produktnummer ABIN8069239

Das Human IgG ELISA Kit (ABIN8069239) ist ein Colorimetric ELISA Kit zur Detektion von Human IgG.

Kurzübersicht für IgG ELISA Kit (ABIN8069239)

Target: IgG

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Serum

Produktdetails IgG ELISA Kit

Verwendungszweck: COVID-19 Alpha Spike Variant Human IgG ELISA Kit 2 is an antibody detection kit (indirect ELISA method) for the quantitative measurement of human IgG against the spike protein mutations (N501Y) associated with the Alpha variant of SARS-CoV-2. This Product is for Research Use Only. Not for use in in vitro diagnostics.

Analytische Methode: Semi-Quantitative

Bestandteile:

• SARS-CoV-2 Protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgG
• HRP-Streptavidin concentrate
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent
• 5X Sample Diluent

,

• SARS-CoV-2 Protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgG
• HRP-Streptavidin concentrate
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent
• 5X Sample Diluent

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Distilled or deionized water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -80 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

Details zu IgG

Target: IgG

Substanzklasse: Antibody

Hintergrund: Gene Symbols: IGG