Fibrinogen ELISA Kit

Produktnummer ABIN8068829

Human Fibrinogen ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Fibrinogen.

Kurzübersicht für Fibrinogen ELISA Kit (ABIN8068829)

Target: Fibrinogen

Bindungsspezifität: citrullinated

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.5625 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Lysate

Produktdetails Fibrinogen ELISA Kit

Untere Nachweisgrenze: 1.5625 ng/mL

Verwendungszweck: Citrullinated Fibrinogen Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Citrullinated Fibrinogen (Fgn).

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human Citrullinated Fibrinogen.

Sensitivität: 0.19 ng/mL

Bestandteile:

Size / Qty
• Anti-Pan-Fgn Microplate
• 96 wells
• Citrullinated-Fgn Standard
• Anti-Citrullinated Fgn Detection Antibody
• HRP-conjugated anti-mouse IgG concentrate
• 25 μL
• Wash Buffer
• Assay Diluent B
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Log-log graph paper or computer and software for ELISA data analysis
• Absorbent paper
• Tubes to prepare positive control or sample dilutions
• Distilled or deionized water
• Shaker

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL of each standard and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-conjugated anti-mouse IgG solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Details zu Fibrinogen

Target: Fibrinogen

Hintergrund:

Gene Symbols: FGB

Background: The Citrullinated Fibrinogen Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Citrullinated Fibrinogen (Fgn). An anti-pan fibrinogen antibody has been coated onto a 96-well plate. Standard and Samples are pipetted into the wells and fibrinogen present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-citrullinated fibrinogen antibody is used to detect Citrullinated Fibrinogen. After washing away unbound antibody, HRP-conjugated anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Citrullinated Fibrinogen bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): Fibrinogen beta chain [Cleaved into: Fibrinopeptide B, Fibrinogen beta chain]

Gen-ID: 2244

UniProt: P02675