Digoxin ELISA Kit

Produktnummer ABIN8068665

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human, Rind (Kuh) und Maus Digoxin.

Kurzübersicht für Digoxin ELISA Kit (ABIN8068665)

Target: Digoxin (DIG)

Reaktivität: Human, Rind (Kuh), Maus

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.01 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Plasma, Saliva, Serum, Urine

Produktdetails Digoxin ELISA Kit

Untere Nachweisgrenze: 0.01 ng/mL

Verwendungszweck: This kit is a competitive immunoassay that quantifies DIG in serum or plasma from human, mouse, rat, equine, and bovine samples, as well as urine samples within 2.5 hours.

Analytische Methode: Quantitative

Spezifität: This EIA kit detects Digoxin

Sensitivität: 0.0427 ng/mL

Bestandteile:

Size / Description
• DIG Microplate
• 96 wells coated with DIG
• Assay Diluent
• Standard DIG
• Positive Control
• Anti-DIG Antibody
• ~10 μL
• HRP-conjugated Secondary Antibody
• ~5 μL
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• GraphPad Prism or SigmaPlot software

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2-4 folds

Probenmenge: 50 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 μL standards or samples to appropriate well. Incubate for 10 minutes at room temperature.
3. Add 50 μL anti-DIG to appropriate well. Incubate 1 hour at room temperature. And wash 5 times.
4. Add 100 μL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature. And wash 5 times.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10-25 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Details zu Digoxin

Target: Digoxin (DIG)

Andere Bezeichnung: Digoxin

Substanzklasse: Chemical

Hintergrund: Optional[Gene Symbols]: Digoxin (DIG) is a once-daily medication used to treat certain heart conditions when first-line therapies are ineffective or when additional treatment is required. Because digoxin has a narrow therapeutic index, careful monitoring is essential. Excessive digoxin levels can lead to dangerous, and sometimes fatal, cardiac arrhythmias. Therefore, serum digoxin testing helps ensure that drug levels are sufficient to provide therapeutic benefit while avoiding toxicity.

The DIG Enzyme Immunoassay (EIA) Kit is a competitive immunoassay that quantifies DIG in serum or plasma from human, mouse, rat, equine, and bovine samples, as well as urine samples within 2.5 hours. Absorbance is measured at 450 nm, and concentrations are interpolated from a ready-to-use DIG standard curve. The assay demonstrates a sensitivity of 0.0427 ng/mL and has been validated for linearity, stability, precision, and spike-recovery performance. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.