COX IV ELISA Kit

Produktnummer ABIN8068573

Das Human COX IV ELISA Kit (ABIN8068573) ist ein Colorimetric ELISA Kit zur Detektion von Human COX IV.

Kurzübersicht für COX IV ELISA Kit (ABIN8068573)

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.28 ng/mL - 80 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails COX IV ELISA Kit

Untere Nachweisgrenze: 0.28 ng/mL

Verwendungszweck: The Human COX4I1 ELISA kit is a pre-coated sandwich-based assay kit designed to quantitatively measure COX4I1 levels in human cell culture media, plasma, and serum.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human COX4I1. Other species not determined.

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)
• Adhesive Sealing Film

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard or sample dilutions
• Plastic wrap

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2-10 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Details zu COX IV

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Andere Bezeichnung: COX4I1

Hintergrund:

Gene Symbols: COX4I1

Protein Name (Synonyms): Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (Cytochrome c oxidase polypeptide IV) (Cytochrome c oxidase subunit IV isoform 1) (COX IV-1)

Gen-ID: 1327

UniProt: P13073

Pathways: Proton Transport