Cortisol ELISA Kit

Produktnummer ABIN8068528

Das Human, Maus und Ratte Cortisol ELISA Kit (ABIN8068528) ist ein Colorimetric ELISA Kit zur Detektion von Human, Maus und Ratte Cortisol.

Kurzübersicht für Cortisol ELISA Kit (ABIN8068528)

Target: Cortisol

Reaktivität: Human, Maus, Ratte

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.1 ng/mL - 1000 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Cortisol ELISA Kit

Untere Nachweisgrenze: 0.1 ng/mL

Verwendungszweck: Human/Mouse/Rat Cortisol EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA

Analytische Methode: Quantitative

Spezifität: This EIA kit is designed to detect human, mouse, and rat Cortisol.

Sensitivität: 2.7 ng/mL

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Standard Peptide
• HRP-Conjugated Peptide
• Capture Antibody
• Positive Control
• Wash Buffer
• Assay Diluent(s)
• TMB One-Step Substrate Reagent
• Stop Solution

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Distilled or deionized water
• Aluminum foil
• Plastic wrap

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: Human: 8x / Mouse: 2x / Rat: 2x

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well. Incubate 1.5 hours at room temperature or overnight at 4 °C.
3. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C.
4. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
5. Add 50 μL Stop Solution to each well. Read plate at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C to -80°C for up to 6 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.

Details zu Cortisol

Target: Cortisol

Substanzklasse: Hormone

Hintergrund: Optional[Gene Symbols]: Cortisol is a naturally occurring pregnane corticosteroid and is also known as 11β,17α,21-trihydroxypregn-4-ene-3,20-dione. Cortisol is synthesized from cholesterol mainlyby the adrenal gland in the zona fasciculata. Release of Cortisolis increased in response to stress and low blood-glucose concentration. It functions to increase blood sugar through gluconeogenesis, to suppress the immune system, and to aid in the metabolism of fat, protein, and carbohydrates. It also decreases bone formation.