CA242 ELISA Kit

Produktnummer ABIN8065412

Human CA242 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human CA242.

Kurzübersicht für CA242 ELISA Kit (ABIN8065412)

Target: CA242 (Carbohydrate Antigen 242 (CA242))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL – 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Produktdetails CA242 ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Verwendungszweck: In vitro quantitative determination of ca242 concentrations in serum, plasma, cell culture supernatant

Analytische Methode: Quantitative

Spezifität: Specifically recognize ca242, no obvious cross reaction with other analogues

Sensitivität: 18.75 pg/mL

Bestandteile: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Benötigtes Material: - Microplate reader (wavelength: 450nm)
- 37 °C incubator
- Automated plate washer
- Precision single and multi-channel pipette and disposable tips
- Clean tubes and Eppendorf tubes
- Deionized or distilled water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Sandwich ELISA, Double Antibody

Probenmenge: 100 μL

Testdauer: 4 h

Plattentyp: Pre-coated

Protokoll: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ca242 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti ca242 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ca242 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ca242 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

Testpräzision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate. CV% = <6%
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.CV%= <7%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Details zu CA242

Target: CA242 (Carbohydrate Antigen 242 (CA242))

Andere Bezeichnung: CA242

Hintergrund: CA242(Pancreatic Carcinoma Markers-CA242)