Kininogen (HMW) CLIA Kit

Produktnummer ABIN8056072

Das Human Kininogen (HMW) ELISA Kit (ABIN8056072) ist ein Chemiluminescent ELISA Kit zur Detektion von Human Kininogen (HMW).

Kurzübersicht für Kininogen (HMW) CLIA Kit (ABIN8056072)

Target: Kininogen (HMW) (Kininogen (High Molecular Weight))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.55 ng/mL - 400 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails Kininogen (HMW) CLIA Kit

Untere Nachweisgrenze: 0.55 ng/mL

Verwendungszweck: CLIA Kit for High Molecular Weight Kininogen (HMWK)

Analytische Methode: Quantitative

Spezifität:

This assay has high sensitivity and excellent specificity for detection of High Molecular Weight Kininogen (HMWK).
No significant cross-reactivity or interference between High Molecular Weight Kininogen (HMWK) and analogues was observed.

Sensitivität: < 0.21 ng/mL

Bestandteile:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• Substrate A
• Substrate B
• Wash Buffer (30X)
• Instruction manual

Benötigtes Material:

• Luminometer capable of reading 96-well microplates with the following parameters: lag time 30.0secs; read time 1.0 sec/well
• Single or multi-channel pipettes with high precision and disposable tips.
• Microcentrifuge Tubes.
• Deionized or distilled water.
• Absorbent paper for blotting the microplate.
• Container for Wash Solution.
• 0.01mol/L (or 1×) Phosphate Buffered Saline(PBS), pH7.0-7.2

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Testpräzision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Molecular Weight Kininogen (HMWK) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Molecular Weight Kininogen (HMWK) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Haltbarkeit: 6 months

Antigendetails

Target: Kininogen (HMW) (Kininogen (High Molecular Weight))

Andere Bezeichnung: High Molecular Weight Kininogen

Hintergrund: Williams-Fitzgerald-Flaujeac Factor, Fitzgerald Factor, HMWK-Kallikrein Factor

UniProt: P01042