MEP1A ELISA Kit

Produktnummer ABIN8055893

Human MEP1A ELISA Kit, Colorimetric Assay für die Quantifizierung von Human MEP1A.

Kurzübersicht für MEP1A ELISA Kit (ABIN8055893)

Target: MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))

Bindungsspezifität: AA 22-601

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails MEP1A ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Verwendungszweck: Sandwich Human MEP1A ELISA Kit to quantitate Human MEP1A in cell culture supernatants, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: V22-Q601

Capture antibody and Detection antibody: polyclonal antibody from goat, biotinylated polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 50 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 7.1%, 4%, 5.4%
Inter-Assay Precision (CV%): 6.6%, 6.7%, 6.7%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Details zu MEP1A

Target: MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))

Andere Bezeichnung: MEP1A

Hintergrund:

Background: Meprin A subunit alpha also known as endopeptidase-2 or PABA peptide hydrolase is the alpha subunit of the meprin A enzyme that in humans is encoded by the MEP1A gene. Meprin A subunit alpha, encoded by the MEP1A gene, is a component of the meprin A metalloproteinase complex, which is involved in extracellular matrix remodeling and proteolysis. Meprin A is a zinc-dependent endopeptidase found predominantly in the kidneys, intestines, and other tissues. It plays a crucial role in various physiological processes, including inflammation, tissue repair, and the processing of bioactive peptides. The alpha subunit, specifically, is a part of the hetero-oligomeric enzyme complex, which combines with the beta subunit to form an active enzyme. Abnormalities in meprin A function have been associated with several pathological conditions, including kidney diseases and cancer.

Gene Full Name: meprin A subunit alpha

Gen-ID: 4224

UniProt: Q16819