NAAA ELISA Kit

Produktnummer ABIN8055851

Human NAAA ELISA Kit, Colorimetric Assay für die Quantifizierung von Human NAAA.

Kurzübersicht für NAAA ELISA Kit (ABIN8055851)

Target: NAAA (N-Acylethanolamine Acid Amidase (NAAA))

Bindungsspezifität: AA 1-359

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Produktdetails NAAA ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: Sandwich Human ASAHL ® Quick ELISA Kit ELISA Kit. Quantitate Human ASAHL in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: CHO
Immunogen sequence: M1-K359

Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 8 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 2.9%, 4.9%, 7.3%
Inter-Assay Precision (CV%): 3.3%, 3.5%, 2.8%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Details zu NAAA

Target: NAAA (N-Acylethanolamine Acid Amidase (NAAA))

Andere Bezeichnung: NAAA

Hintergrund:

Background: N-acylethanolamine-hydrolyzing acid amidase is an enzyme that in humans is encoded by the NAAA gene. Enables N-(long-chain-acyl)ethanolamine deacylase activity, N-acylsphingosine amidohydrolase activity, and fatty acid amide hydrolase activity. Involved in several processes, including N-acylethanolamine metabolic process, N-acylphosphatidylethanolamine metabolic process, and sphingosine metabolic process. Located in lysosome. Is extrinsic component of membrane.

Gene Full Name: N-acylethanolamine acid amidase

Gen-ID: 27163

UniProt: Q02083