AGRN ELISA Kit

Produktnummer ABIN8055423

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Ratte AGRN.

Kurzübersicht für AGRN ELISA Kit (ABIN8055423)

Target: AGRN (Agrin (AGRN))

Bindungsspezifität: AA 1153-1959

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails AGRN ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Sandwich Rat Agrin ELISA Kit to quantitate Rat Agrn in cell culture supernatants, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: A1153-P1959

Capture antibody and Detection antibody: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 4.2%, 4.9%, 5.1%
Inter-Assay Precision (CV%): 5.6%, 6.4%, 7.1%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Details zu AGRN

Target: AGRN (Agrin (AGRN))

Andere Bezeichnung: Agrn

Hintergrund:

Background: Agrin is a large proteoglycan whose best-characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis. In humans, this protein is encoded by the AGRN gene.This protein has nine domains homologous to protease inhibitors.It may also have functions in other tissues and during other stages of development. It is a major proteoglycan component in the glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions.

Gene Full Name: agrin

Gen-ID: 25592

UniProt: P25304

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development