NAD+/NADH Colorimetric Assay Kit (WST-8)

Produktnummer ABIN7850058

Kurzübersicht für NAD+/NADH Colorimetric Assay Kit (WST-8) (ABIN7850058)

Target: NAD/NADH

Nachweismethode: Colorimetric

Applikation: Biochemical Assay (BCA)

Proben: Cell Samples, Tissue Samples

Analytische Methode: Quantitative

Produktdetails

Detektionsbereich: 0.02 μmol/L - 5.0 μmol/L

Untere Nachweisgrenze: 0.02 μmol/L

Verwendungszweck: NAD+/NADH Colorimetric Assay Kit (WST-8)

Produktmerkmale: Detect total content of NAD+ and NADH Ethanol generates acetaldehyde under the action of enzyme. Meanwhile, NAD+ is reduced to NADH, NADH, under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NAD+ and NADH can be quantified by measure the OD value at 450 nm. Detect NADH After treating sample, heat at 60oC water bath for 30 min. the NAD+ of the sample is decomposed and only NADH remains. NADH reduces WST-8 to form yellow product, and the amount of NADH is determined by measure the OD value at 450 nm. Detect NAD+ and NAD+/NADH The content of NAD+ and the ratio of NAD+/NADH in the sample can be obtained according to the total content of NAD+ and NADH obtained of the first two steps as well as the separate content of NADPH. Note: NADP+ and NADPH have no effect on the determination results.

Anwendungsinformationen

Testdauer: 1 h

Testpräzision:

Inter-assay precision: 9.1%

Intra-assay precision: 1.8%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect from light.

Lagerung: -20 °C

Informationen zur Lagerung: This product can be stored at -20°C for 12 months with shading light.

Haltbarkeit: 12 months

Antigendetails

Target: NAD/NADH

Andere Bezeichnung: NAD+/NADH

Substanzklasse: Chemical