CPB2 ELISA Kit
Human CPB2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human CPB2.
Produktnummer ABIN7832955
769,23 €
Zzgl. Versandkosten 20,00 € und MwSt
Lieferung in 8 bis 9 Werktagen
Kurzübersicht für CPB2 ELISA Kit (ABIN7832955)
Target
Alle CPB2 ELISA Kits anzeigen
CPB2
(Carboxypeptidase B2 (Plasma) (CPB2))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
1.56 ng/mL - 100 ng/mL
Applikation
ELISA
Proben
Cell Culture Supernatant, Plasma, Serum
-
-
Untere Nachweisgrenze
-
1.56 ng/mL
-
Verwendungszweck
-
Sandwich ELISA for quantitative detection of Human TAFI concentrations.
-
Analytische Methode
-
Quantitative
-
Sensitivität
-
670 pg/mL
-
Bestandteile
-
- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
-
Benötigtes Material
-
- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
-
-
-
Probenmenge
-
100 μL
-
Plattentyp
-
Pre-coated
-
Protokoll
-
- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
-
Beschränkungen
-
Nur für Forschungszwecke einsetzbar
-
-
Handhabung
-
Avoid freeze/thaw cycles
-
Lagerung
-
4 °C,-20 °C
-
Informationen zur Lagerung
-
Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.
-
Haltbarkeit
-
12 months
-
-
CPB2
(Carboxypeptidase B2 (Plasma) (CPB2))
-
Andere Bezeichnung
-
CPB2
-
Hintergrund
-
Alternative Names: Carboxypeptidase B2, Carboxypeptidase U, CPU, Plasma carboxypeptidase B, pCPB, Thrombin-activable fibrinolysis inhibitor, TAFI
-
Gen-ID
-
1361
-
UniProt
-
Q96IY4
-
Pathways
-
Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis
-