CD47 ELISA Kit
Kurzübersicht für CD47 ELISA Kit (ABIN7832952)
Target
Alle CD47 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 312 pg/mL
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Verwendungszweck
- Sandwich ELISA for quantitative detection of Human CD47 concentrations.
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Analytische Methode
- Quantitative
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Sensitivität
- 116 pg/mL
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Bestandteile
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- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
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Benötigtes Material
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- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
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CD47 (CD47) ELISA Kit
CD47 Reaktivität: Human Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate, Urine
CD47 (CD47) ELISA KitCD47 Reaktivität: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
CD47 (CD47) ELISA KitCD47 Reaktivität: Maus Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
CD47 (CD47) ELISA KitCD47 Reaktivität: Human Colorimetric Sandwich ELISA 3.12-200 pg/mL Plasma, Serum, Tissue Homogenate, Urine
CD47 (CD47) ELISA KitCD47 Reaktivität: Human AA 19-139 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Serum
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Probenmenge
- 100 μL
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Plattentyp
- Pre-coated
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Protokoll
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- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- Avoid freeze/thaw cycles
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.
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Haltbarkeit
- 12 months
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- CD47
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Andere Bezeichnung
- CD47
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Hintergrund
- Alternative Names: MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47
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Gen-ID
- 961
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UniProt
- Q08722
Target Alle CD47 ELISA Kits anzeigen
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