alpha Fetoprotein ELISA Kit
Kurzübersicht für alpha Fetoprotein ELISA Kit (ABIN7832945)
Target
Alle alpha Fetoprotein (AFP) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 781 pg/mL
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Verwendungszweck
- Sandwich ELISA for quantitative detection of Human Alpha-fetoglobulin concentrations.
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Analytische Methode
- Quantitative
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Sensitivität
- 280 pg/mL
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Bestandteile
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- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
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Benötigtes Material
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- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
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alpha-Fetoprotein (AFP) ELISA Kit
AFP Reaktivität: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-Fetoprotein (AFP) ELISA KitAFP Reaktivität: Human Colorimetric Sandwich ELISA 1.563-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
alpha-Fetoprotein (AFP) ELISA KitAFP Reaktivität: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
alpha-Fetoprotein (AFP) ELISA KitAFP Reaktivität: Maus Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
alpha-Fetoprotein (AFP) ELISA KitAFP Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
alpha-Fetoprotein (AFP) ELISA KitAFP Reaktivität: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
alpha-Fetoprotein (AFP) ELISA KitAFP Reaktivität: Maus Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
alpha-Fetoprotein (AFP) ELISA KitAFP Reaktivität: Huhn Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
alpha-Fetoprotein (AFP) ELISA KitAFP Reaktivität: Hund Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-Fetoprotein (AFP) ELISA KitAFP Reaktivität: Schwein Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Probenmenge
- 100 μL
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Plattentyp
- Pre-coated
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Protokoll
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- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- Avoid freeze/thaw cycles
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.
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Haltbarkeit
- 12 months
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- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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Andere Bezeichnung
- AFP
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Hintergrund
- Alternative Names: HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin
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Gen-ID
- 174
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UniProt
- P02771
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Pathways
- C21-Steroid Hormone Metabolic Process
Target Alle alpha Fetoprotein (AFP) ELISA Kits anzeigen
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