VEGF 165 ELISA Kit

Produktnummer ABIN7832884

Human VEGF 165 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human VEGF 165.

Kurzübersicht für VEGF 165 ELISA Kit (ABIN7832884)

Target: VEGF 165

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Plasma, Serum

Güteklasse: QuickTest

Produktdetails

Verwendungszweck: FastELISA VEGF165 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human VEGF165 (Vascular Endothelial Growth Factor 165) in Serum, plasma.

Analytische Methode: Quantitative

Spezifität: This assay is highly specific for detection of Human VEGF165. No significant cross-reactivity or interference between Human VEGF165 and analogues was observed.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
2. Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
3. Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freezing and thawing

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.

Haltbarkeit: 6 months

Antigendetails

Target: VEGF 165

Andere Bezeichnung: VEGF165