VCAM1 ELISA Kit
Kurzübersicht für VCAM1 ELISA Kit (ABIN7832883)
Target
Alle VCAM1 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
Güteklasse
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Verwendungszweck
- FastELISA VCAM1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human VCAM1 (Vascular Cell Adhesion Molecule 1) in Serum, plasma.
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Analytische Methode
- Quantitative
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Spezifität
- This assay is highly specific for detection of Human VCAM1. No significant cross-reactivity or interference between Human VCAM1 and analogues was observed.
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Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit
VCAM1 Reaktivität: Human Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reaktivität: Maus Colorimetric Sandwich ELISA 39.062-2500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reaktivität: Ratte Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reaktivität: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reaktivität: Kaninchen Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reaktivität: Schwein Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reaktivität: Human AA 25-698 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reaktivität: Hund Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reaktivität: Affe Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Plattentyp
- Pre-coated
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Protokoll
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- Avoid repeated freezing and thawing
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Haltbarkeit
- 6 months
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- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
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Andere Bezeichnung
- VCAM1
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Hintergrund
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Synonyms: CD106|DKFZp779G2333|INCAM-100|MGC99561
Gene Description: vascular cell adhesion molecule 1
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Gen-ID
- 7412
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Pathways
- Carbohydrate Homeostasis
Target Alle VCAM1 ELISA Kits anzeigen
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