ADIPOQ ELISA Kit
Kurzübersicht für ADIPOQ ELISA Kit (ABIN7832851)
Target
Alle ADIPOQ ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
Güteklasse
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Verwendungszweck
- FastELISA ADIPOQ (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human ADIPOQ (Adiponectin) in Serum, plasma.
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Analytische Methode
- Quantitative
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Spezifität
- This assay is highly specific for detection of Human ADIPOQ. No significant cross-reactivity or interference between Human ADIPOQ and analogues was observed.
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Adiponectin (ADIPOQ) ELISA Kit
ADIPOQ Reaktivität: Human Colorimetric Sandwich ELISA 1.562-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reaktivität: Maus Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reaktivität: Human Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reaktivität: Schaf Colorimetric Competition ELISA 6.25 ng/mL - 100 ng/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reaktivität: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reaktivität: Kaninchen Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reaktivität: Huhn Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reaktivität: Schwein Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reaktivität: Human AA 19-244 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate, Urine
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Plattentyp
- Pre-coated
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Protokoll
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- Avoid repeated freezing and thawing
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Haltbarkeit
- 6 months
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- ADIPOQ (Adiponectin (ADIPOQ))
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Andere Bezeichnung
- ADIPOQ
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Hintergrund
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Synonyms: ACDC|ACRP30|ADPN|APM-1|APM1|GBP28|adiponectin
Gene Description: adiponectin, C1Q and collagen domain containing
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Gen-ID
- 9370
Target Alle ADIPOQ ELISA Kits anzeigen
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