ICAM1 ELISA Kit
Kurzübersicht für ICAM1 ELISA Kit (ABIN7832841)
Target
Alle ICAM1 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
Güteklasse
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Verwendungszweck
- FastELISA ICAM-1/CD54 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human ICAM-1/CD54 (intercellular adhesion molecule 1) in serum, plasma and other biological fluids.
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Analytische Methode
- Quantitative
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Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit
ICAM1 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reaktivität: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reaktivität: Kaninchen Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reaktivität: Hund Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reaktivität: Schwein Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reaktivität: Human AA 28-480 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reaktivität: Maus AA 28-485 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reaktivität: Ratte AA 28-493 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reaktivität: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Plattentyp
- Pre-coated
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Protokoll
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- Avoid repeated freezing and thawing
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 2-8C for 6 months.Avoid repeated freezing and thawing.
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Haltbarkeit
- 6 months
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- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
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Andere Bezeichnung
- ICAM-1/CD54
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
Target Alle ICAM1 ELISA Kits anzeigen
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